Identification of optimal measurement sets for complete flux elucidation in metabolic flux analysis experiments
نویسندگان
چکیده
منابع مشابه
Identification of optimal measurement sets for complete flux elucidation in metabolic flux analysis experiments.
Metabolic flux analysis (MFA) methods use external flux and isotopic measurements to quantify the magnitude of metabolic flows in metabolic networks. A key question in this analysis is choosing a set of measurements that is capable of yielding a unique flux distribution (identifiability). In this article, we introduce an optimization-based framework that uses incidence structure analysis to det...
متن کاملFlux-P: Automating Metabolic Flux Analysis
Quantitative knowledge of intracellular fluxes in metabolic networks is invaluable for inferring metabolic system behavior and the design principles of biological systems. However, intracellular reaction rates can not often be calculated directly but have to be estimated; for instance, via 13C-based metabolic flux analysis, a model-based interpretation of stable carbon isotope patterns in inter...
متن کاملOptimal flux patterns in cellular metabolic networks.
The availability of whole-cell-level metabolic networks of high quality has made it possible to develop a predictive understanding of bacterial metabolism. Using the optimization framework of flux balance analysis, I investigate the metabolic response and activity patterns to variations in the availability of nutrient and chemical factors such as oxygen and ammonia by simulating 30,000 random c...
متن کاملIsotopomer measurement techniques in metabolic flux analysis II: mass spectrometry.
Mass spectrometry (MS) offers a sensitive, reliable, and highly accurate method for measurement of isotopic labeling, which is required for generating comprehensive flux maps using metabolic flux analysis (MFA). We present protocols for assessing isotope labeling in a wide range of biochemical species, including proteinogenic amino acids, free organic and amino acids, sugar phosphates, lipids, ...
متن کاملNonstationary Metabolic Flux Analysis (NMFA) for the Elucidation of Cellular Physiology by
Many current and future applications of biological engineering hinge on our ability to measure, understand, and manipulate metabolism. Many diseases for which we seek cures are metabolic in nature. Small-molecule biomanufacturing almost always involves metabolic engineering. Biofuels, a current topic of great interest, is essentially a metabolic problem. Even bioprocesses that involve complex p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2008
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.21926